Gab2遺伝子のノックアウトマウス。Gab2はアダプター分子であり、様々なチロシンキナーゼによってリン酸化を受ける。リン酸化されたチロシン残基はSH2ドメインを有するPI-3キナーゼやSHP-2といったシグナル伝達分子と会合する。Gab2欠損マウスでは、マスト細胞の発生障害が観察され、アナフィラキシーに対して、抵抗性を示す。マウスではGab1とGab2のファミリー分子が報告されている。サイトカイン・増殖因子を介するシグナル伝達の解析、アレルギーに関する研究に有用。 mouse phosphoglycerate kinase promoter, E. coli Neomycin resistance gene, mouse Gab2 genomic DNA R1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>] 大阪大学大学院医学系研究科・平野俊夫（2002）。R1 ES細胞を用いて作出。C57BL/6と129の混合背景。 RBRC02821 B6;129-Gab2<tm1Thir> B6;129-Gab2<tm1Thir> 平野　俊夫 条件を付加する。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。Blood, 99, 1866-1869 (2002).<br>提供にあたっては寄託者の承諾を必要とする。 非営利機関が非営利目的の教育・研究用に用いる場合以外は、大阪大学と別途 MTA を締結すること。 Gab2 gene knockout mice. Exon encoding a major portion of the PH domain was replaced with a neo cassette. Gab2 gene is an adaptor molecule that plays a critical role in mediating cellular transformation. The number of mast cells is severely reduced in the stomach and less severely in the skin of homozygous mutant mice. Bone marrow-derived mast cells from the homozygous mutant mice exhibit an impaired KitL-induced ERK MAP kinase and Akt activation. true Gab2 KO mouse Gab2 KO mouse Heterozygote x Homozygote [or Crossing to C57BL/6JJcl] Reproductive performance is not so good. Heterozygote x Homozygote [or Crossing to C57BL/6JJcl] Reproductive performance is not so good. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Blood, 99, 1866-1869 (2002).The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER. The RECPIENT who wants to use the BIOLOGICAL RESOURCE for the purpose other than education and research in non-profit organization is requested to make a Material Transfer Agreement with Osaka University. Immunology and Inflammation Research Toshio HIRANO C（3〜6か月） C (3-6 months) Cell Biology Research Developed by Toshio Hirano, Osaka University Graduate School of Medicine in 2002. R1 ES cells were used. C57BL/6 and 129 mixed background. <a href='https://brc.riken.jp/mus/pcr02821'>Genotyping protocol -PCR-</a> Necessary documents for ordering:<ol><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol><A HREF="https://www.med.osaka-u.ac.jp/pub/molonc/www/Eindex.html" target="_blank">Lab HP</A>